FEATURES
Olympus IX71 inverted manual stand with motorized xy-stage and fast objective Z-piezo (travel range 100 μm)
Spinning disk confocal microscope for fast live cell imaging
Stage-top incubator for heating and CO2 level control for 35mm dishes and chambered slides
Suitable for imaging multi-well plates, 35 mm dishes, and (chambered) slides
Suitable for confocal imaging, photo-bleaching and -activation with high temporal and spatial resolution
ILLUMINATION
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
Transmitted light (Halogen)
Fluorescence (HBO)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
Andor Micropoint:
Scanner-based coupled in through back port
405nm pulsed dye laser (15 Hz pulse frequency)
FILTERS
Stand:
Reflector revolver (manual): pos1: GFP (BP 470/40, LP 500, X HQ530/30); pos2: RFP (BP 568/10, LP 598, BP 630/62); pos3: DAPI HC FilterSet (377/50 BL HC, HC 409, 447/60 BL HC); pos4: cutter DC (z375 RDC, R 375, T 319-800); pos5: empty; pos6: DIC analyzer
CSU-X scan head:
Dichromatic mirror slider: Quad band T-405/488/568/647, Triple band T-405/488/561, Single band T-488 (all from Semrock)
Emission filter wheel (RotR):
pos0: 624/40; pos1: empty, pos2: BP 525/30 BL HC; pos3: RazorEdge LP Edge Filter 561 RU; pos4: DBP 512/23 + 630/91 HC; pos5: RazorEdge LP Edge Filter 488 LP; pos6: old CSU dual emitter: 525/30 + 650/?; pos7: BP 605/70 ET; pos8: BP 445/40 BL HC; pos9: Edge Basic 635LP
DETECTION
Andor iXon Ultra 897 EMCCD, Monochrome EMCCD camera (SN 3329)
Dexel (detector element) size: 16 μm
Andor Neo sCMOS, Monochrome sCMOS camera (SN 0898)
Dexel (detector element) size: 6.5 μm
OBJECTIVES
Olympus U Plan SApo 10x (0.4 NA) Air
Olympus U PlanFluar 20x (0.5 NA) Air
Olympus U Apo 40x (1.15 NA) Water
Olympus U PlanFluarN 40x (0.75 NA) Air
Olympus U Plan SApo 60x (1.2 NA) Water
Olympus U Plan ApoN 60x (1.42 NA) Oil
Olympus U Plan SApo 100x (1.4 NA) Oil
More information about the pixel sizes and FOV can be found here
SCAN HEAD
SOFTWARE
Andor iQ 3.6