Light Microscopy

MPI-CBG > Research > Scientific Cores & Support > Scientific Services > Light Microscopy > Super-resolution Microscopes > H5
NIKON TIRF FRAP (INTERNAL NAME – H5)

FEATURES

  • Nikon Eclipse Ti2 inverted motorized stand with hardware autofocus (PFS)

  • Stage-top Z-piezo (travel range 100μm) 

  • Scanner-based photo-manipulation (FRAP, PA, FLIP...)

  • Automated water immersion dispenser

  • Cage incubator for heating, CO2 & O2 level control 

  • Suitable for fast dual-color TIRF, long-term focus-stabilized low-light level imaging

  • Suitable for superresolution localisation microscopy (PALM, STORM…)

  • Suitable for imaging multi-well plates, 35 mm dishes, and (chambered) slides

ILLUMINATION

  • Laser diode 405 nm 

  • Laser DPSS 488 nm

  • Laser DPSS 561 nm

  • Laser DPSS 640 nm

  • Transmitted light (LED)

  • Fluorescence (SPECTRA III light engine):

    • 399/22 nm

    • 440/20 nm

    • 475/28 nm

    • 510/25 nm

    • 555/28 nm

    • 575/25 nm

    • 635/22 nm

    • 740/20 nm

  • More information about the laser power measurements can be found here

FILTERS

  • Ti2 Stand:

    • Reflector revolver FL1 (upper floor): 

      • pos1:  TIRF DM cube (quad DC: zt405/488/561/640rpc)

      • pos2:  TIRF DM Quad EM cube (quad DC: zt405/488/561/640rpc + quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28)

      • pos3: empty

      • pos4: analyser

      • pos5: LED Penta EX & DM

      • pos6: LED Triple EX & DM

    • Emission wheel 1 (lower floor): 

      • pos1: 515/30

      • pos2: 525/50

      • pos3: 605/40

      • pos4: 680/42

      • pos5: Penta EM

      • pos6: Triple EM

      • pos7: empty 

  • Gemini dichromatic mirrors:

    • LP 488

    • LP 514

    • LP 561

    • LP 643

  • Gemini emission filters:

    • Dual 445/30 + 528/35

    • Dual 589/37 + 700/60

    • 435/40

    • 475/35

    • 525/50

    • 542/27

    • 595/50

    • 607/36

    • 685/70

    • 810/90

DETECTION

  • pco.panda 4.2M sCMOS camera (PN: 70108074001; SN 14403660)

  • Andor iXon DU-888U3-CSO- #BV EMCCD (SN X-14458)

  • Hamatsu ORACA- Fusion BT sCMOS (Model C15440 - 20UP, SN 501389; "camera 1")

  • Hamatsu ORACA- Fusion BT sCMOS  (Model C15440 - 20UP, SN 501392; "camera 2")

OBJECTIVES

  • Nikon Plan Apochromat Lambda D 4x (0.2 NA) Air; WD: 20mm

  • Nikon Plan Apochromat Lambda D 10x (0.45 NA) Air DIC N1; WD: 4mm

  • Nikon Apochromat Lambda S 40x (1.25 NA) Water DIC N2; WD: 0.20 - 0.16mm

  • Nikon Plan Apochromat 60x (1.27 NA) Water DIC N2 WD: 0.18 - 0.16mm

  • Nikon Apochromat 100x (1.49 NA) Oil DIC N2 WD: 0.16 - 0.09mm; for TIRF; with automated correction collar

SOFTWARE

  • NIS-Elements 5.42.05 (Build 1817)