Light Microscopy

MPI-CBG > Research > Scientific Cores & Support > Scientific Services > Light Microscopy > Laser Scanning Confocal Microscopes > CZ6

ZEISS LSM 880 AIRY INVERTED (INTERNAL NAME – CZ6)

FEATURES

  • Zeiss Axio Observer.Z1 inverted stand with stage-top piezo (travel range 100 um)

  • Single photon point scanning confocal system with a Quasar detector (32 spectral detection channels in the GaAsP detector plus 2 PMTs) and transmitted light detector

  • Airy scan detector for high resolution imaging and high speed imaging

  • Motorized stage: suitable for 2D images, z-stacks, time-lapses, bleaching and tile scans

  • UV 355nm laser for photomanipulaiton and imaging

  • Photomanipulation (PA/FRAP/FLIP/FLAP)

  • Stage-top and/or cage incubator, environmental control of heating, and CO2

  • Suitable for live samples, fixed samples and tissue sections

  • Suitable for slides, chambered slides, 35 mm dishes, and multi-well plates

ILLUMINATION

  • Laser 355 nm

  • Laser 405 nm

  • Argon laser multiline 458, 488, 514 nm

  • Laser 561 nm

  • Laser 594 nm

  • Laser 633 nm

  • Transmitted light (halogen)

  • Fluorescence (metal halide, HXP 120 W)

  • More information about the laser power measurements can be found here

PHOTOMANIPULATION

  • All visible laser lines through main scanner

FILTERS

  • Stand: 

    • Reflector revolver: Cy3.5 (Zeiss set 49); DAPI (Ex G365, BS FT 395, EM BP 445/50); GFP (Zeiss set 38); Endow GFP shift free (E) (EX BP 470/40, BS FT 495, EM BP 525/50); HQ Texas Red (Zeiss set 45) shift free (E) (EX BP 560/40, BS FT 585, EM BP 630/75)

  • Scan head: 

    • MBS Twingate Visible branch: 458, 458/514, 458/514/594, 488, 488/561, 488/561/633, T80/R20, Plate

    • MBS Twingate Invisible branch: Plate, T80/R20, 355, 355/445, LP405, LP445

    • Airyscan filters: 420-480/495-550, 420-480/495-620, 420-480 / LP 605, 465-505 / LP525, 495-550 / LP570, 570-620 / LP645

DETECTION

  • 32 GaAsP PMT (photomultiplier) channels 

  • 2 PMTs

  • 1 32-channel Airy scan detector

  • Transmission detection (T-PMT)

OBJECTIVES

  • Zeiss Plan Apo 10x (0.45 NA) Air

  • Zeiss Plan Apo 20x (0.8 NA) Air

  • Zeiss LD LCI Plan Apo 25x (0.8 NA) W / Gly / Oil DIC

  • Zeiss C-Apo 40x (1.2 NA) Water

  • Zeiss Plan Apo 63x (1.4 NA) Oil DIC

  • Zeiss LCI Plan-Neofluar 63x (1.3 NA) Imm Korr DIC

  • Zeiss Plan Apo 40x (0.95) Korr

SOFTWARE

  • Zeiss ZEN 2.3 SP1 FP3 (black) version 14.0.25.201