FEATURES
Olympus IX81 inverted motorized stand with hardware autofocus (ZDC1) and stage-top Z-piezo (travel range 400 μm)
Spinning disk confocal microscope for fast live cell imaging
Scanner-based photo-manipulation (FRAP, PA, FLIP...)
Stage-top incubator for heating / cooling and CO2 level control for 35 mm dishes only
Suitable for imaging multi-well plates, 35 mm dishes, and (chambered) slides
Suitable for confocal imaging, photo-bleaching and -activation with high temporal and spatial resolution
ILLUMINATION
Laser diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
Transmitted light (LED)
Fluorescence (HBO)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
Andor FRAPPA:
Scanner-based coupled in through imaging port
With all 4 imaging laser lines (fast switching between imaging and photomanipulation)
FILTERS
Stand:
Reflector revolver: pos1: empty; pos2: DIC analyzer; pos3: GFP/mCherry Dualband ET FilterSet (F56-019); pos4: GFP ET FilterSet (470/40 ET BP, T 495 LPXR, ET BP525/50); pos5: mCherry FilterSet (560/40 ET BP, T 585 LPXR, 630/75 ET BP); pos6: DAPI HC BP FilterSet (377/50 BL HC, HC 409, 447/60 BL HC)
CSU-X scan head:
Dichromatic mirror slider: Quad band T-405/488/568/647, Triple band T-405/488/561, Single band T-488
Emission filter wheels:
Sutter filter wheel A: pos0: 440/521/607/700 HC Quadband; pos1: RazorEdge LP Edge Filter 488 LP; pos2: 708/75 BL HC; pos3: RazorEdge LP Edge Basic Filter 568; pos4: RazorEdge LP Edge Basic Filter 635; pos5: BP 445/45 BL HC; pos6: empty; pos7: polarizer for DIC; pos8: BP 525/30 BL HC; pos9: BP 607/36 BL HC
Sutter filter wheel B: pos0: empty; pos1: BP 470/22 BL HC; pos2-8: empty; pos9: BP 586/25 HC
DETECTION
Andor iXon 897 EMCCD, Monochrome EMCCD camera (SN 3880)
Dexel (detector element) size: 16 μm
Andor Clara CCD (SN 01156)
Dexel (detector element) size: 6.45 μm
OBJECTIVES
Olympus U Plan SApo 10x (0.4 NA) Air
Olympus U Apo 40x (1.15 NA) Water
Olympus U Plan SApo 60x (1.2 NA) Water
Olympus U Plan SApo 60x (1.35 NA) Oil
Olympus U Plan SApo 100x (1.4 NA) Oil
More information about the pixel sizes and FOV can be found here
SCAN HEAD
Yokogawa CSU-X1 (5000 rpm, pinhole diameter 50 μm, pinhole spacing 250 μm)
SOFTWARE
Andor iQ 3.6