FEATURES
Zeiss Axio Observer.Z1 inverted motorized stand with stage-top Z-piezo (travel range 100 μm)
Spinning disk confocal microscope for fast live cell imaging
Rapp OptoElectronic setup for additional scanner-based photo-manipulation (FRAP: point- and ROI-scanning; and UV-laser ablation)
Stage-top incubator heating and cooling of 35 mm dishes and CO2 level control
Suitable for imaging multi-well plates, 35 mm dishes and (chambered) slides without incubation
ILLUMINATION
Laser diode 405 nm
Laser DPSS 445 nm
Laser DPSS 488 nm
Laser DPSS 514 nm
Laser DPSS 561 nm
Laser DPSS 639 nm
Transmitted light (LED)
Fluorescence (metal halide, HXP, 120 W)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
RAPP Optoelectronic:
Scanner-based coupled in through back port
355 nm laser for ablation (1 kHz rep rate, max 42 μJ pulse power, 1 ns pulse length)
405 and 473 nm lasers for photobleaching and photoactivation
4-line LDI for scanned ROI photomanipulation (405, 470, 555 and 640 nm)
FILTERS
Stand - Reflector Revolver:
pos1: empty for use with CSU
pos2: Ex 365, BS 395, EM BP 445/50 (Zeiss FS49)
pos3: Ex BP470/40, BS 495, EM BP 525/50 (Zeiss FS38HE)
pos4: TDC 355/405/473 (zt 355/405/473 rpc) for RAPP photomanipulation
pos5: Analyser DIC
pos6: T387LP for RAPP photomanipulation
additional spare filter cubes:
Ex BP550/25, BS 570, EM BP605/70 (Zeiss FS 43 HE)
Ex BP640/30, BS660, EM BP690/50 (Zeiss FS50)
TFT80/20 for RAPP photomanipulation
T555LP for RAPP photomanipulation
CSU-X1 scan head:
Dichromatic mirrors:
RTFT 405/488/561 - Triple
RQFT 405/488/568/647 - Quad
RTFT 457/514/647 - Triple
Emission filter wheels:
Zeiss AxioCam 705 mono with EM filter wheel (FW1):
pos1: BP 450/50 ET
pos2: BP 485/30 ET
pos3: DBP 527/54 + 654/60 (Zeiss FS61HE)
pos4: BP 535/30 ET
pos5: BP 629/56 BL HC
pos6: BP 690/50 ET
Photometrics Evolve EMCCD with EM filter wheel (FW2):
pos1: DBP 527/54 + 645/60 (Zeiss FS61HE)
pos2: DBP 480/22 + LP530 (Zeiss FS59HE)
pos3: LP 655 ET
pos4: BP 525/50 ET
pos5: BP 600/50 ET
pos6: empty
DETECTION
Zeiss AxioCam 705 Mono, CCD camera (SN 136210640)
Photometrics Evolve, Monochrome EMCCD camera (SN A11B103006)
OBJECTIVES
Air objectives:
Zeiss EC Plan Neofluar 5x (0.16 NA) Air Ph1
Zeiss Plan Apochromat10x (0.45 NA) Air
Zeiss Plan Apochromat 20x (0.8 NA) Air
Zeiss Plan LD Achroplan 63x (0.75 NA) Air Ph2
Zeiss EC Plan Neofluar 63x (0.95 NA) Air
Multi-immersion objectives:
Zeiss LD LCI Plan Apochronmat 25x (0.8 NA) Oil/Gly/Water DIC
Zeiss LCI Plan Neofluar 63x (1.3 NA) Gly/Water DIC
Water immersion objectives:
Zeiss C-Apochromat 63x (1.2 NA) Water
Oil immersion objectives:
Zeiss Plan Apochromat 40x (1.3 NA) Oil DIC
Zeiss Plan Apochromat 63x (1.4 NA) Oil
Zeiss alpha-Plan Fluar 100x (1.45x) Oil DIC
Zeiss alpha-Plan Apo 100x (1.46 NA) Oil DIC
Pixel sizes and FOV:
More information about the pixel sizes and FOV can be found here
SCAN HEAD
Yokogawa CSU-X1 (5000 rpm, pinhole diameter 50 μm, pinhole spacing 250 μm)
SOFTWARE
Zeiss ZEN 3.2 Blue
For RAPP: SysCon2