FEATURES
Telight inverted motorized two-arm stand (reference and object) with stage-top Z-piezo (travel range 500 μm)
Multi-modal, low-coherence interference-based microscope for label-free quantitative phase transmitted light imaging (QPI) that may be combined with 3 channels of fluorescence imaging
Motorized XYZ-stage and cage incubator for live cell imaging
Ideal for measurement of ‘dry-mass of cells’ in monolayers, protein concentration in biomolecular condensates, as well as refractive index mapping in layers of known thickness
ILLUMINATION
650 nm LED for low-coherence transmitted light QPI
Lumencore AURA III for fluorescence
FILTERS
Stand: Excitation Lumencore: DAPI (390/22), FITC (475/28), TRITC (555/28)
Multi-band dichroic DAPI/FITC/TRITC Semrock FF403/497/574-Di01
Single emission filters: DAPI (Semrock FF02-435/40), FITC (Semrock FF02-520/28), TRITC (Semrock FF02-617/73)
DETECTION
2 Andor Zyla 4.2, sCMOS, USB 3
OBJECTIVES
Nikon CFI Plan Apochromat 4x (0.1 NA) Air
Nikon CFI Plan Fluor 10x (0.3 NA) Air
Nikon CFI Plan Fluor 20x (0.5 NA) Air
Nikon CFI Plan Apochromat 40x (0.95 NA) Air
Nikon CFI Plan Apochromat 60x (1.4 NA) Oil
SOFTWARE
SophiQ version 9.2 (includes automated segmentation of individual cells for subsequent data analysis)