Light Microscopy

MPI-CBG > Forschung > Serviceeinheiten > Wissenschaftlicher Service > Light Microscopy > Spinning Disk Confocal Microscopes > SD5
OLYMPUS IXPLORE SPINSR SPINNING DISK (INTERNAL NAME – SD5)

FEATURES

  • Olympus IX83 inverted motorized stand with hardware autofocus (ZDC2) and stage-top Z-piezo (travel range 400 μm) 

  • Spinning disk confocal microscope for fast live cell, organoid, and large area imaging with high spatial resolution (down to 120 nm in XY) via SORA disk

  • Photoactivation using 405 nm laser with high temporal and spatial resolution.

  • Rapp Optoelectronics FLUCS  photomanipulation system

  • Cage incubator for heating, CO2, and O2 level control

  • Suitable for imaging multi-well plates, 35 mm dishes, and chambered slides

 

ILLUMINATION

  • Laser diode 405 n

  • Laser diode 445 nm

  • Laser DPSS 488 nm

  • Laser DPSS 515 nm

  • Laser DPSS 561 nm

  • Laser DPSS 640 nm

  • Transmitted light (LED)

  • Fluorescence (LED: CoolLED pE-4000 with 16 lines: pE-4000 | LED Light Sources | CoolLED LED Illumination System)

  • More information about the laser power measurements can be found here

PHOTOMANIPULATION

  • RAPP Optoelectronic FLUCS:

    • Scanner-based coupled in through back port

    • 1455 nm for focus-light induced cytoplasmic streaming (FLUCS)

  • FRAP:

    • Scanner-based through right side port 

    • Laser diode 405 nm

FILTERS

  • Stand: 

    • Reflector revolver:

      • pos1: STLP-FLUCS-RAPP

      • pos2: U-FUNA (Ex 360-370, DM 410, EM 420-460)

      • pos3: U-FBNA (Ex 470-495, DM 505, EM 510-550)

      • pos4: U-FGW (Ex 530-550, DM 570, EM 575IF)

      • pos5: none

      • pos6: TxRed ET Filterset (Ex 560/40, DM 585, EM 630/75, installed 4th April 2023; before: 405_LED_FR)

      • pos7: Cy5 ET Filterset (Ex 620/60, DM 660, EM 700/75, installed 4th April 2023; before: DCSP1260-FLUCS-AHF)

      • pos8: IX3-FDICT (DIC analyzer)

  • CSU-W scan head:

    • Dichromatic mirrors:

      • Quad 405/488/561/640

      • Triple 445/514/640

      • full mirror

    • Dichromatic beamsplitter:

      • Glass plate

      • 561 LP

      • 514 LP

  • Emission filter wheels:

    • Emission filter wheel 1:

      • pos1: BP 447/60 BL HC (DAPI)

      • pos2: BP 525/50 BL HC (GFP, Alexa 488)

      • pos3: BP 617/73 BL HC (RFP, Cherry wide)

      • pos4: BP 685/40 BL HC (Cy5, SIR)

      • pos5: BP 482/35 BL HC (CFP)

      • pos6: BP 578/105 BL HC (YFP, Venus)

      • pos7: BP 607/36 BL HC (RFP, Cherry narrow)

      • pos8: LP Edge Basic 647 (BLP01-647R-25)

      • pos 9: LP Edge Basic 488 (BLP01-488R-25)

      • pos10: LP Edge Basic 568 (BLP01-568R-25)

    • Emission filter wheel 2:

      • pos1: BP 447/60 BL HC (DAPI)

      • pos2: BP 525/50 BL HC (GFP, Alexa 488)

      • pos 3: BP 482/35 BL HC (CFP)

      • pos4: BP 542/30 BL HC (YFP, Venus)

      • pos5: BP 514/30 BL HC (narrow GFP)

      • pos 6: polarizer

DETECTION

  • 2 Hamamatsu ORCA-Fusion BT Digital CMOS cameras (SN 500674 and SN 500670)

    • Dexel (detector element) size: 6.5 μm

OBJECTIVES

  • Olympus U Plan SApo 4x (0.16 NA) Air

  • Olympus U Plan SApo 10x (0.4 NA) Air

  • Olympus U Plan XApo 20x (0.8 NA) Air

  • Olympus U Plan SApo 20x (0.85 NA) Oil

  • Olympus U Plan SApo IR 30x (1.05 NA) Sil

  • Olympus U Plan CN 40x (0.65 NA) Air

  • Olympus U Plan SApo 40x (0.95 NA) Air

  • Olympus U Plan 60x (1.2 NA) Water

  • Olympus U Plan SApo 60x (1.3 NA) Sil

  • Olympus U Plan SApo 100x (1.35 NA) Sil

  • Olympus U-ApoN 100x (1.49 NA) Oil

  • More information about the pixel sizes and FOV can be found here

SCAN HEAD

  • Yokogawa CSU-W1 (4000 rpm with two disks: 50 μm and SORA for superresolution) 

SOFTWARE

  • Olympus cellSens 4.1

  • For RAPP: SysCon2