FEATURES
Leica DMI6000 inverted stand
Combined single- and multiphoton imaging equipped with an 8 kHz resonant / galvo tandem scanner
Multiphoton excitation by two laser lines: one tunable from 680 nm-1300 nm and one fixed at 1040 nm
Suitable for photomanipulation (PA, FRAP, FLIP, FLAP, laser cutting/ablation…)
Suitable for slides, chambered slides, 35 mm dishes, and multi-well plates
Suitable for fixed samples, live samples and tissue sections
Stage-top incubator for control of heating and CO2 from Okolab
ILLUMINATION
Laser diode 405 nm
Laser solid state 488 nm
Laser solid state 552 nm
Laser solid state 638 nm
Laser Multiphoton Spectra Physics InSight Deep See Dual (one tunable line: 680-1300 nm, one fixed line 1041 nm)
Fluorescence (metal halide)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
All visible laser lines through main scanner
FILTERS
Stand:
Reflector revolver: DAPI; GFP/RFP
Scan head:
BS: Substrate, DD448/514, DD488/552, TD 488/552/638, TD 448/514/552, RT 15/85
MPF: Substrate, SP815, SP665, Mirror
NDD filter for 2P imaging: BP 617/73, BP 525/50, BP 700/75, BP 460/50
DETECTION
Confocal: spectral detector with 5 detectors: 4 hybrid detectors (HyD) and 1 PMT
Multiphoton: 4 non-descanned detectors (NDDs): 2 HyDs and 2 PMTs
OBJECTIVES
Leica HC PL FLuotar 5x Air
Leica HC CS Plan Apo 10x (0.4 NA) Air
Leica HC CS Plan Apo 20x (0.7 NA) Air
Leica HC CS2 Plan Apo 20x (0.75 NA) Imm
Leica HCX IRApo L 25x (0.95 NA) Water
Leica HC CS PL Apo 40x (0.85 NA) Air
Leica HC PL Apo 40x (0.85 NA) Corr Air
Leica HC PL IRApo 40x (1.1 NA) Water
Leica HC PL Apo CS2 40x (1.25 NA) Glyc 23/37 °C
Leica HC CS2 Plan Apo 63x (1.3 NA) Glyc 23/37°C
Leica HC PL Apo CS2 63x (1.4 NA) Oil
SOFTWARE
LasX 3.5.7.23225