FEATURES
Olympus IX83 inverted motorized stand with stage-top Z-piezo (travel range 400 μm)
Spinning disk confocal microscope for fast live cell, organoid, and large area imaging
Equipped with Borealis for even illumination
Photoactivation using 405 nm laser with high temporal and spatial resolution
Cage incubator for heating, CO2, and O2 level control
Suitable for imaging multi-well plates, 35 mm dishes, and (chambered) slides
ILLUMINATION
two connected Andor laser combiner ILE (integrated laser engine)
Laser diode 405 nm
Laser diode 445 nm
Laser DPSS 488 nm
Laser DPSS 514 nm
Laser DPSS 561 nm
Laser DPSS 633 nm
Laser DPSS 726 nm
Transmitted light (LED)
Fluorescence (LED)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
Andor Mosaic
DMD-based coupled in through back port
Laser diode 405 nm (450 mW)
FILTERS
Stand:
Reflector revolver:
position 1: none
position 2: DAPI set (Ex 377/50, DM 409, EM 447/60)
position 3: GFP set (Ex 472/30, DM 495, EM 520/35)
position 4: RFP set (Ex 562/40, DM 593, EM 624/40)
position 5: CY5 set (Ex 628/40, DM 660, EM 692/40)
position 6: Analyzer
position 7: none
position 8: DM for use with Mosaic (DM LP405)
CSU-W1 scan head:
Primary dichromatic mirrors (main beam splitter):
Triple 445/514/640
Quad 405/488/561/640
Penta 405/488/561/640/720
Secondary dichromatic mirrors (image splitter):
561LP
514LP
glass plate
Emission filter wheels:
Emission filter wheel 1 (primary camera):
pos1: RazorEdge LP Edge Basic Filter 568
pos2: BP 525/50 BL HC
pos3: BP 617/73 BL HC
pos4: BP 607/36 BL HC
pos5: BP 685/40 BL HC
pos6: BP 785/62 BL HC
pos7: polarizer
pos8: RazorEdge LP Edge Basic Filter 514
pos9: BP 542/27 BL HC
pos10: BP 452/45 BL HC
Emission filter wheel 2 (secondary camera):
pos1: BP 465/30 BL HC
pos2: BP 514/30 BL HC
pos3: BP 525/50 BL HC
pos4: BP 479/40 BL HC
pos5: polarizer
pos6–10: empty
DETECTION
2 Andor iXon 888 Ultra EMCCD cameras (with etaloning suppression):
primary: SN DU888_BV (SN X-10332)
secondary: SN DU888_BV (SN X-10396)
Dexel (detector element) size of both cameras: 13 μm
OBJECTIVES
Olympus U Plan SApo 10x (0.4 NA) Air
Olympus U Plan Fluar 20x (0.5 NA) Air
Olympus U Plan SApo 30x (1.05 NA) Sil
Olympus U Plan SApo 40x (1.25 NA) Sil
Olympus U Plan SApo 60x (1.3 NA) Sil
Olympus U Plan SApo 100x (1.4 NA) Oil
Olympus U Plan SApo 100x (1.34 NA) Sil
Olympus U ApoN 150x (1.45 NA) TIRFM
More information about the pixel sizes and FOV can be found here
SCAN HEAD
Yokogawa CSU-W1:
4000 rpm
two pinhole disks:
25 μm diameter
50 μm diameter
pinhole spacing: 500 μm (for both disks)
Borealis illumination delivery
SOFTWARE
Andor iQ 3.6