Sequencing and Genotyping

MPI-CBG > Forschung > Serviceeinheiten > Wissenschaftlicher Service > Sequencing and Genotyping > Services

Next generation sequencing

We are offering a broad range of different sequencing applications and help and guide our users to find the most efficient and econimic sequencing technology to answer their scientific questions. Our services cover routine NGS-based workflows as well as customized sequencing solutions.

Long read sequencing and associated technologies 

We are applying different long read DNA and RNA sequencing, haplotyping, and scaffolding technologies to create de novo genome assemblies, improve upon existing assemblies, resolve highly-repetitive regions and annotate repeat and gene regions.

Services

  • Single molecule long read PacBio sequencing of genomes, RNA Isoforms, long amplicons, long targeted genomic regions (in development)
  • Single molecule long read Oxford Nanopore sequencing of genomes, RNA, long amplicons
  • Bionano optical mapping of genomes to improve genome assemblies or detect large structural variants in genomes
  • Chromatin confirmation capture protocols to analyse long intra-chromosomal interactions
  • Haplotyping of long genomic regions by linked genomic reads (Tell-Seq protocol)
  • High-quality de novo genome assembly pipeline for complex high-quality genomes
  • Basic genome annotation pipeline based on RNAseq and protein data

Short read and single cell sequencing

Many short-read applications involving Illumina sequencing are routinely performed by our colleagues in the DcGC. You are always welcome to get in touch with us and we will connect you with our experts.

Services

  • RNA sequencing (from bulk tissues and single cells)
  • ATAC seq (from bulk tissues and single cells)
  • Targeted re-sequencing
  • Whole genome bisulfite sequencing
  • ChIP-sequencing
  • TCR profiling

Extraction of genomic DNA

Our DNA extraction portfolio ranges from rapid protocols for fast genotyping applications to the extraction of ultra-long and contaminant-free genomic DNA that is required for high-quality de novo genome assemblies. We have experience with different input including bacteria, plants, small animals, cultured cell, and extracted tissues of different species over multiple taxa.

We have also different electrophoresis systems in place that allow the separation of long and ultra-long gDNA fragments.

We are happy to discuss and help to find the best and most economical way of DNA extraction depending on your sample type and input amount always taking your follow up application into account.

Services

  • Rapid gDNA lysates (different protocols for different species)
  • Extraction of high-quality genomic DNA (kits of different suppliers)
  • Extraction of long and ultra-long genomic DNA (> 200 kb)
  • Post-purification of genomic DNA for highly sensitive single molecule sequencing technologies
  • Separation of long DNA fragments by pulse-field gel electrophoresis (PFGE)
  • High-throughput capillary gel-electrophoresis

Genotyping

We provide a service that designs and executes pipelines to characterize molecular events in genome engineering projects and a standard genotyping service of genetic model organisms.

Services      

  • Routine genotyping of model organisms (mouse, zebrafish, fly) by PCR-based methods.
  • Characterization of CRISPR-Cas9 induced sequence variations in collaboration with our Genome engineering facility.