A microsphere is moved with optical tweezers by a well-defined pattern.
Optical trap pong game
Using a dual optical trap to play pong with two beads. Movie by Michael Bugiel (BCF)
Rotation of microtubule-coated beads
Widefield fluorescence imaging of optically trapped, microtubule-coated beads that rotated under flow. Movie by Serapion Pyrpassopoulos (Schaeffer group, Uni Tuebingen)
C. elegans embryo
Lattice lightsheet, max. projection. Movie by Okafornta William (Müller-Reichert group)
Volvox
XWing scope, movie by Robert Haase (Myers group), sample by Mangal Prakash (Jug group)
C. elegans embryo, His-GFP βTub-GFP
Bessel SPIM, movie by Loïc Royer
Bryozoan larva
Optical trap screen saver
Minimum projection of moving bead with optical tweezers.
Optical trap writing
Controlled manipulation of a microsphere for writing. Movie by Michael Bugiel (BCF)
Force-dependent labeling of DNA
Widefield fluorescence imaging of DNA which is optically trapped with two beads. At higher loads, more sytox dye binds to the DNA. Movie by Pranay Mandal (Grill group)
Micropatterning of TEM grids
Selective passivation of PEGylated TEM grids by micropatterning, visualized by GFP binding. Image by Petra Kiesel (von Appen group)
Micropatterning on a fluorescent coverslide
“Writing” the MPI logo by micropatterning on a fluorescent coverslide. Image by M. Bugiel (BCF)
Zebrafish mitosis
Lattice lightsheet. Max. proj. of an histone-GFP labelled Zebrafish embryo.
Drosophila embryo, His-RFP
XWing scope, movie by Robert Haase (Myers group)
Zebrafish brain acute manipulation. Ras-GFP
Bessel SPIM. Ablation: 920 nm, 500 mW, Ti:Sa laser. Sample by Jaroslav Icha, Imaging by Kei Murata
Multiphoton imaging of Oscarella
Excitation 940 nm. Sample by Emmanuel Reynaud
Optical trap writing
Controlled manipulation of a microsphere for writing. Movie by Michael Bugiel (BCF)
DNA-coated beads
Widefield fluorescence imaging of optically trapped, DNA-coated beads. Image by Franziska Lesche (Brugues group)
Nanoindentation of a gastruloid
Indenting a gastruloid with 26 um sphere. Image by Marc Trani (Veenvliet lab).
Nanoindentation of soft samples
Nanoindenter measurement with indentation, relaxation, and retraction. Sample by Niyousha Baboli (Tabler lab).
Insulin granules
Lattice lightsheet (max projection), 3 volumes/second. Movie by Andreas Müller (Solimena group)
3-D reconstruction of endosomes (green) and mitochondria (red) in a HeLa cell
