We apply shotgun mass spectrometry technology to full-lipidome analyses to acquire the full lipidome profile of organs, tissues, bio-fluids or in vitro models obtaining insights of several biomedical conditions. Our main focus is on human and mice liver, gut (colon), brain and plasma embracing various health conditions (from metabolic disorders to cancer).
Among our main projects, we are interested in liver pathological conditions, such as non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) since the lipid accumulation in hepatocytes, and the additional fibrotic changes happen during NASH.
We aim to investigate the role of genetic risk markers of NAFLD in liver lipid metabolism by performing liver lipidome. Establishing clinically-relevant reference values (in moles) of the healthy liver lipidome, its changes in obese patients, and patients with NAFLD and NASH is an essential part of translational aspect of current work.
Our main collaborators for full-lipidome studies: Dr. Sebastian Zeissig’s lab (Center for Regenerative Therapies, Dresden); Dr. Jochen Hampe’s lab (University Hospital, Dresden); Dr. Ünal Coskun’s lab (Paul Langerhans Institute, Dresden); Liver Systems Medicine (LiSyM) research network; Dr. Wieland Huttner’s lab (MPI-CBG, Dresden).
Veera Raghavan Thangapandi, Oskar Knittelfelder, Mario Brosch, Eleonora Patsenker, Olga Vvedenskaya, Stephan Buch, Sebastian Hinz, Alexander Hendricks, Marina Nati, Alexander Herrmann, Devavrat Ravindra Rekhade, Thomas Berg, Madlen Matz-Soja, Klaus Huse, Edda Klipp, Josch K Pauling, Judith Ah Wodke, Jacobo Miranda Ackerman, Malte von Bonin, Elmar Aigner, Christian Datz, Witigo von Schönfels, Sophie Nehring, Sebastian Zeissig, Christoph Röcken, Andreas Dahl, Trian Chavakis, Felix Stickel, Andrej Shevchenko, Clemens Schafmayer, Jochen Hampe, Pallavi Subramanian
Loss of hepatic Mboat7 leads to liver fibrosis.
Gut, Art. No. doi: 10.1136/gutjnl-2020-320853 (2020) DOI
Yuting Wang, Sebastian Hinz, Ortrud Uckermann, Pia Hönscheid, Witigo von Schönfels, Greta Burmeister, Alexander Hendricks, Jacobo Miranda Ackerman, Gustavo Baretton, Jochen Hampe, Mario Brosch, Clemens Schafmayer, Andrej Shevchenko, Sebastian Zeissig
Shotgun lipidomics-based characterization of the landscape of lipid metabolism in colorectal cancer.
Biochim Biophys Acta Mol Cell Biol Lipids, 1865(3) Art. No. 158579 (2020) DOI
Olga Vvedenskaya, Yuting Wang, Jacobo Miranda Ackerman, Oskar Knittelfelder, Andrej Shevchenko
Analytical challenges in human plasma lipidomics: A winding path towards the truth
Trends Analyt Chem, Art. No. 115277 (2018) DOI
Susanne Sales, Juergen Graessler, Sara Ciucci, Rania Al-Atrib, Terhi Vihervaara, Kai Schuhmann, Dimple Kauhanen, Marko Sysi-Aho, Stefan Bornstein, Marc Bickle, Carlo Vittorio Cannistraci, Kim Ekroos, Andrej Shevchenko
Gender, Contraceptives and Individual Metabolic Predisposition Shape a Healthy Plasma Lipidome.
Sci Rep, 6 Art. No. 27710 (2016) PDF DOI
Susanne Sales, Oskar Knittelfelder, Andrej Shevchenko
Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry.
Methods Mol Biol, 1619 203-212 (2017) DOI
Santosh Phuyal, Mayes Kasem, Oskar Knittelfelder, Animesh Sharma, Davi de Miranda Fonseca, Vaineta Vebraite, Sergey Shaposhnikov, Geir Slupphaug, Vidar Skaug, Shanbeh Zienolddiny
Characterization of the proteome and lipidome profiles of human lung cells after low dose and chronic exposure to multiwalled carbon nanotubes.
Nanotoxicology, 12(2) 138-152 (2018) DOI
Oskar Knittelfelder, Sofia Traikov, Olga Vvedenskaya, Andrea Schuhmann, Sandra Segeletz, Anna Shevchenko, Andrej Shevchenko
Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool To Analyze Histological Zones in Cryosections of Tissues.
Anal Chem, 90(16) 9868-9878 (2018) DOI
