Cell line Knock-Out
- Guide RNA design and synthesis
- Complete general cell culture work
- Generating cell lines by electroporation
- Picking single colonies of cells or cell line clone
- generation by FACS
- Sequencing including sequence validation
Cell line Knock-In
- Guide RNA design and synthesis
- Complete general cell culture work
- HDR template design and synthesis
- Generating cell lines by electroporation
- Picking single colonies of cells or cell line clone generation by FACS
- Sequencing including sequence validation
iPS cell Knock-Out
- Guide RNA design and synthesis
- Complete general iPS cell culture work
- Generating cell lines by electroporation
- Picking single colonies of cells
- Sequencing including sequence validation
iPS cell Knock-In
- Guide RNA design and synthesis
- Complete general iPS cell culture work
- HDR template design and synthesis
- Generating cell lines by electroporation
- Picking single colonies of cells
- Sequencing including sequence validation