C. elegans genome editing by microinjection
C.elegans Knock-Out strain
- Guide RNA design and synthesis
- In vivo injection
- Worm picking and screening
- Genotyping
- Sequencing including sequence validation
- Freezing and Remp storage
C.elegans tagging
- Ordering of 2x HDR templates-Guide RNA design and synthesis
- In vivo injection
- Worm picking and screening
- Genotyping
- Fluorescence microscopy
- Sequencing including sequence validation
- Freezing and Remp storage