Services: MPI-CBG

single particles and macromolecules (liposome, proteinosome, microtubles ...)
tissue cultured cells (HeLa, MDCK, MEF, RPE, human pancreatic progenitor cells, human fibroblast…)
single cell organisms (bacteria, yeast, chlamydomonas, diatomes…)
various tissues from model systems
- Drosophila
- flatworms
- C.elegans
- zebrafish
- mouse
- organoids and spheroids (brain, pancreas, liver …)
- others …

Fixation
- Chemical fixation
- Physical fixation
  - Plunge freezing
  - High Pressure Freezing (in combination with Freeze substitution)
Embedding
- For morphological study
  - TEM samples
  - SEM samples
- For functional and morphological study (immunolabeling)
  - Tokuyasu technique [technique link] [example link]
  - Embedding in acrylic resin (at low temperature)
Ultramicrotomy
- room temperature ultramicrotomy
  - semithin sectioning (500 nm-2 um, with Toluidinblue Staining)
  - ultrathin sectioning (50-70 nm)
  - serial sectioning
  - sectioning for TEM tomography (300 nm)
  - Ultramicrotomy in SBF-SEM (3ViewXP)
  - FIB lamellae (under development)
- cryo ultramicrotomy
  - Tokuyasu sectioing (for immunolabelling)
  - CEMOVIS (under development)
  - FIB lamellae (under development)
Immuno EM
- Pre embedding labelling (DAB, ultrasmall gold labelling with silver enhancement)
- On-grid (post-embedding) labelling
  - on Tokuyasu sections
  - on plastic (methacrylate) sections
  - with negative contrasting (for isolated molecules or organelles)
CLEM (correlative light and electron microscopy)
- room temperature light microscopic (LM) features with RT-TEM structure
- room temperature LM features (endogenous or immunlabelling) with immunoEM
- room temparature LM features with cryoEM
- CryoLM with cryoEM (under development)
negative staining
- can be used for single particles, macromolecular complexes, organelles, can be combined with immunogoldlabelling

Electron Microscopy