The knockdown efficiency is an important feature for any silencing trigger. Because of the complex mixture of different siRNAs all targeting the same mRNA, the esiRNA pool typically produces excellent silencing of the transcripts.

QRT-PCR analyses 24 hours after transfection of HeLa cells with indicated esiRNAs are shown.

Western blot analyses 72 hours after transfection of HeLa with indicated esiRNAs are shown.