Biological mass spectrometry is a major analytical tool to study the interplay between molecular composition, spatial organization and dynamics of complex biological systems.
The three pillars of our work - lipidomics, proteomics and analytical technology development – are bundled by absolute (molar) quantification of a broad palette of biomolecules.
Molar quantities of proteins and lipids elucidate the stoichiometry of molecular assemblies from protein complexes to organelles and organs; characterize their dynamics during organism development as well as organism adaptation to dietary and environmental challenges. Absolute quantification provides reference values of protein and lipid disease markers in liquid and solid biopsies and is highly relevant for clinical chemistry and diagnostics.
Method
Software
Method
Absolute quantification of fluorescent proteins and their fusions
Detecting rare translational errors by data-independent acquisition
MS Western: The Method for Targeted Multiplexed Absolute Quantification of Proteins by GeLC-MS/MS
Proteomics by Data-Independent Acquisition
Fast-tracking QconCAT approach for absolute protein quantification